| 64 | Jill van Den Dungen (SBU) def. Marija Leko (GV-W) 6-2, 6-1 |
| 64 | Ashley Jonathan (BELL) def. Emily Sim (TUWOMEN) 4-6, 6-2, 10-7 |
| 64 | Alina Kislitskaya (INDY) def. Linda Liong (WSUW) 7-5, 6-3 |
| 64 | Laiying Alvarado (UF) def. Isabela Paixao (FS) 6-1, 6-2 |
| 64 | Viktoriya Zheleva (DRURY) def. Joie Reisch (LU) 6-3, 6-2 |
| 64 | Maria Kacalova (WALSHW) def. Lena van Bergen (PNW) 6-1, 6-1 |
| 64 | Ivona Gorgioski (MTW) def. Willemien Coetzee (DU) 6-2, 6-1 |
| 64 | Hannah Burth (UMSL) def. Katie Bell (HCW) 7-6, 6-2 |
| 64 | Dominika Bobik (MTW) def. Vera Griva (GV-W) 6-7, 7-5, 10-8 |
| 64 | Maria Solnyshkina (INDY) def. Sarah Hackman (HCW) 7-5, 7-6 |
| 64 | Odette Beagrie (UMSL) def. Joanne Gao (SV) 6-3, 6-4 |
| 64 | Catarina Zheng (SBU) def. Agata Klak (FS) 7-6, 1-6, 10-2 |
| 64 | Pafundi, Nicole (MUW) def. Gysell Daza Gutierre (SV) 6-0, 6-1 |
| 64 | Ava Ratcliff (BELL) def. Nadege Koua (LSSU) 3-6, 6-2, 10-5 |
| 64 | Mercedes Fernandez (WALSHW) def. Carolina Yepez (PNW) 6-2, 6-3 |
| 64 | Khadija Da-Silva (WSUW) def. Maegan Flight (UIS) 7-5, 6-2 |
| 64 | Maja Pindelska (INDY) def. Constansa Crespo (SBU) 4-6, 6-3, 10-4 |
| 64 | Alma Abazi (DRURY) def. Jennifer Skonieczny (BELL) 6-2, 6-3 |
| 64 | Lizaveta Demchenko (INDY) def. Hinterberger, Sandra (MUW) 6-0, 7-5 |
| 64 | Brittany Lavenant (FS) def. Aleksandra Sredojevi (LU) 6-4, 4-6, 10-6 |
| 64 | Anna Novikova (INDY) def. Neva Manas (MTW) 6-0, 6-0 |
| 64 | Taylor Barrett (GV-W) def. Maddie Miller (SV) 6-3, 6-4 |
| 64 | Abbie-Jade Riley (TUWOMEN) def. AlexiAnn Drouin (LSSU) 6-2, 6-3 |
| 64 | Teodora Radu (WSUW) def. Luciana Pereyra (UF) 6-0, 6-1 |
| 64 | Marina Prediger (UMSL) def. Aubrey Smith (BELL) 6-4, 6-0 |
| 64 | Maria Ugarte (DRURY) def. Danica Malan (DU) 6-3, 4-6, 10-3 |
| 64 | Laura Cazacu (WSUW) def. Silvia Verebes (FS) 6-4, 5-7, 10-3 |
| 64 | Marily Canellopoulos (GV-W) def. Bascunana, Clara (MUW) 6-1, 6-4 |
| 64 | Ashley Long (UIS) def. Olivia Kjaer (PNW) 6-1, 6-0 |
| 64 | Nikol Alekseeva (INDY) def. Nicole Beidacki (WALSHW) 6-0, 6-2 |
| 64 | Samantha Sweet (UF) def. Taylor McLaughlin (SV) 6-3, 6-2 |
| 64-B | Sabrina Laynes (WSUW) def. Kayley Gimbel (UF) 6-0, 6-0 |
| 64-B | Sam Stephenson (FS) def. Nicole Johnson (SV) 6-4, 6-2 |
| 64-B | Maja Lietzau (UMSL) def. Evans, Abbie (CED) 6-4, 1-6, 10-7 |
| 64-B | Eliza Kirov (UF) def. Elli Formentin (HCW) 6-1, 6-0 |
| 64-B | Liv Hanover (GV-W) def. Samantha Johnson (MTW) 6-1, 6-1 |
| 64-B | Lauren Prince (TUWOMEN) def. Keegan Malpass (LSSU) 6-0, 6-2 |
| 64-B | Jovana Vujanic (LU) def. Dowden, Ashley (MUW) 4-6, 6-1, 10-5 |
| 64-B | Lucia Pineiro (UIS) def. Vladislava Shumilova (DU) 6-1, 1-0, retired |
| 64-B | Madison Devillez (BELL) def. Ellie Chawner (HCW) 6-2, 6-2 |
| 64-B | Madison Arquette (UF) def. English, Janelle (CED) 6-0, 6-2 |
| 64-B | Chloe Weihs (INDY) def. Johanna Frye (FS) 6-2, 4-6, 13-11 |
| 64-B | Mirna Golac (MTW) def. Cupper, Georgina (MUW) 6-3, 6-1 |
| 64-B | Kseniia Eremina (DRURY) def. Clara Drasch (WALSHW) 6-3, 6-4 |
| 64-B | Tanja Skalda (WSUW) def. Emma Philippus (DU) 6-0, 6-0 |
| 64-B | Canela Luna (HCW) def. Bianca Buie (UMSL) 7-5, 1-6, 10-8 |
| 64-B | Adeline Nelson (DU) def. English, Liana (CED) 7-5, 5-7, 10-7 |
| 64-B | Maja Kozerski (BELL) def. Kyleigh Henthorn (WALSHW) 6-1, 6-4 |
| 64-B | Kamryn Matthews (HCW) def. Sara Neves (SV) 6-3, 6-1 |
| 64-B | Clara Kuehnle (DRURY) def. Jasmine Cortez (UIS) 6-2, 6-0 |
| 64-B | Morgan Waller (FS) def. Pinto, Thamara (MUW) 6-2, 1-6, 10-4 |
| 64-B | Cristina Nieto (UF) def. Agata Szewczuk (GV-W) 6-7, 6-3, 10-1 |
| 64-B | Anais Geslin (TUWOMEN) def. Sophie Davis (LU) 6-1, 6-0 |
| 64-B | Tatum Matthews (HCW) def. Sophie Gossett (DU) 7-6, 2-6, 12-10 |
| 64-B | Leticia Lunge (UMSL) def. Lillian Mouw (DU) 6-0, 6-0 |
| 64-B | Carla Jewell (WSUW) def. Channing Varnum (BELL) 6-3, 6-3 |
| 64-B | Laura Brito (UF) def. Sofia Khairutdinova (WALSHW) 6-3, 6-3 |
| 64-B | Colvee, Helena (MUW) def. Clara Lopez Bueno (UMSL) 6-7, 6-0, 10-4 |
| 64-B | Anna Valiaeva (INDY) def. Sophia Spinazze (HCW) 6-2, 6-0 |
| 64-B | Hollis, Lauren (CED) def. Madelyn Groulx (FS) 6-0, 6-2 |
| 64-B | Lauren Opalewski (MTW) def. Riley Gilman (LSSU) 6-0, 6-1 |
| 64-B | Taylor Lighty (UF) def. Adiya Young (SV) 6-2, 6-3 |
| 32 | Ashley Jonathan (BELL) def. Jill van Den Dungen (SBU) 4-6, 7-6, 10-5 |
| 32 | Alina Kislitskaya (INDY) def. Laiying Alvarado (UF) 6-1, 6-4 |
| 32 | Viktoriya Zheleva (DRURY) def. Maria Kacalova (WALSHW) 6-3, 6-3 |
| 32 | Ivona Gorgioski (MTW) def. Hannah Burth (UMSL) 6-1, 6-0 |
| 32 | Maria Solnyshkina (INDY) def. Dominika Bobik (MTW) 6-4, 6-1 |
| 32 | Catarina Zheng (SBU) def. Odette Beagrie (UMSL) 6-1, 4-6, 11-9 |
| 32 | Ava Ratcliff (BELL) def. Pafundi, Nicole (MUW) 6-1, 2-6, 15-13 |
| 32 | Khadija Da-Silva (WSUW) def. Mercedes Fernandez (WALSHW) 6-4, 7-6 |
| 32 | Maja Pindelska (INDY) def. Alma Abazi (DRURY) 6-1, 6-3 |
| 32 | Lizaveta Demchenko (INDY) def. Brittany Lavenant (FS) 6-1, 6-2 |
| 32 | Anna Novikova (INDY) def. Taylor Barrett (GV-W) 6-2, 6-1 |
| 32 | Abbie-Jade Riley (TUWOMEN) def. Teodora Radu (WSUW) 6-3, 6-3 |
| 32 | Marina Prediger (UMSL) def. Maria Ugarte (DRURY) 6-1, 6-1 |
| 32 | Laura Cazacu (WSUW) def. Marily Canellopoulos (GV-W) 6-2, 6-3 |
| 32 | Diana Cabrera (SBU) def. Ashley Long (UIS) 6-0, 6-2 |
| 32 | Nikol Alekseeva (INDY) def. Samantha Sweet (UF) 6-2, 6-1 |
| 32-B | Sabrina Laynes (WSUW) def. Sam Stephenson (FS) 6-4, 4-6, 10-5 |
| 32-B | Eliza Kirov (UF) def. Maja Lietzau (UMSL) 7-5, 6-1 |
| 32-B | Liv Hanover (GV-W) def. Lauren Prince (TUWOMEN) 6-3, 6-2 |
| 32-B | Jovana Vujanic (LU) def. Lucia Pineiro (UIS) 6-4, 6-3 |
| 32-B | Madison Devillez (BELL) def. Madison Arquette (UF) 4-6, 6-3, 10-4 |
| 32-B | Chloe Weihs (INDY) def. Mirna Golac (MTW) 7-5, 6-4 |
| 32-B | Kseniia Eremina (DRURY) def. Tanja Skalda (WSUW) 7-5, 6-3 |
| 32-B | Canela Luna (HCW) def. Julia Yanes-Barrera (PNW) 6-1, 6-4 |
| 32-B | Maja Kozerski (BELL) def. Adeline Nelson (DU) 6-1, 6-1 |
| 32-B | Clara Kuehnle (DRURY) def. Kamryn Matthews (HCW) 6-4, 3-6, 11-9 |
| 32-B | Pinto, Thamara (MUW) def. Cristina Nieto (UF) 6-2, 6-3 |
| 32-B | Anais Geslin (TUWOMEN) def. Tatum Matthews (HCW) 6-0, 6-2 |
| 32-B | Carla Jewell (WSUW) def. Leticia Lunge (UMSL) 6-4, 6-4 |
| 32-B | Laura Brito (UF) def. Colvee, Helena (MUW) 6-4, 6-4 |
| 32-B | Anna Valiaeva (INDY) def. Hollis, Lauren (CED) 6-1, 6-4 |
| 32-B | Lauren Opalewski (MTW) def. Taylor Lighty (UF) 6-1, 6-3 |
| 16 | Alina Kislitskaya (INDY) def. Ashley Jonathan (BELL) 6-2, 6-4 |
| 16 | Viktoriya Zheleva (DRURY) def. Ivona Gorgioski (MTW) 6-1, 6-0 |
| 16 | Maria Solnyshkina (INDY) def. Catarina Zheng (SBU) 7-5, 6-4 |
| 16 | Khadija Da-Silva (WSUW) def. Ava Ratcliff (BELL) 6-4, 6-3 |
| 16 | Maja Pindelska (INDY) def. Lizaveta Demchenko (INDY) 6-3, 6-1 |
| 16 | Anna Novikova (INDY) def. Abbie-Jade Riley (TUWOMEN) 3-6, 6-4, 10-6 |
| 16 | Marina Prediger (UMSL) def. Laura Cazacu (WSUW) 7-5, 7-5 |
| 16 | Nikol Alekseeva (INDY) def. Diana Cabrera (SBU) 6-1, 6-3 |
| 16-B | Sabrina Laynes (WSUW) def. Eliza Kirov (UF) 7-6, 6-2 |
| 16-B | Liv Hanover (GV-W) def. Jovana Vujanic (LU) 3-6, 6-2, 10-1 |
| 16-B | Madison Devillez (BELL) def. Chloe Weihs (INDY) 6-4, 6-3 |
| 16-B | Kseniia Eremina (DRURY) def. Canela Luna (HCW) 6-4, 6-0 |
| 16-B | Clara Kuehnle (DRURY) def. Maja Kozerski (BELL) 6-4, 6-2 |
| 16-B | Anais Geslin (TUWOMEN) def. Pinto, Thamara (MUW) 7-6, 6-1 |
| 16-B | Laura Brito (UF) def. Carla Jewell (WSUW) 3-4, retired |
| 16-B | Anna Valiaeva (INDY) def. Lauren Opalewski (MTW) 6-4, 6-4 |
| QF | Alina Kislitskaya (INDY) def. Viktoriya Zheleva (DRURY) 6-1, 6-0 |
| QF | Khadija Da-Silva (WSUW) def. Maria Solnyshkina (INDY) 7-6, 6-2 |
| QF | Anna Novikova (INDY) def. Maja Pindelska (INDY) 6-2, 6-3 |
| QF | Nikol Alekseeva (INDY) def. Marina Prediger (UMSL) 6-4, 2-6, 10-3 |
| QF-B | Sabrina Laynes (WSUW) def. Liv Hanover (GV-W) 6-2, 2-6, 10-2 |
| QF-B | Kseniia Eremina (DRURY) def. Madison Devillez (BELL) 6-2, retired |
| QF-B | Anais Geslin (TUWOMEN) def. Clara Kuehnle (DRURY) 6-1, 6-2 |
| QF-B | Anna Valiaeva (INDY) def. Laura Brito (UF) 7-6, 6-0 |
| SF | Alina Kislitskaya (INDY) def. Khadija Da-Silva (WSUW) 6-2, 6-0 |
| SF | Nikol Alekseeva (INDY) def. Anna Novikova (INDY) 6-3, 6-4 |
| SF-B | Sabrina Laynes (WSUW) def. Kseniia Eremina (DRURY) by default |
| SF-B | Anna Valiaeva (INDY) def. Anais Geslin (TUWOMEN) 6-4, 6-2 |
| F | Nikol Alekseeva (INDY) def. Alina Kislitskaya (INDY) 6-4, 6-7, 10-8 |
| F-B | Anna Valiaeva (INDY) def. Sabrina Laynes (WSUW) 6-4, 6-2 |
| 32-A-C | Emily Sim (TUWOMEN) def. Marija Leko (GV-W) 6-2, 6-2 |
| 32-A-C | Linda Liong (WSUW) def. Isabela Paixao (FS) 6-1, 6-3 |
| 32-A-C | Joie Reisch (LU) def. Lena van Bergen (PNW) 3-6, 6-3, 10-2 |
| 32-A-C | Katie Bell (HCW) def. Willemien Coetzee (DU) 6-2, 6-2 |
| 32-A-C | Vera Griva (GV-W) def. Sarah Hackman (HCW) 6-7, 6-3, 10-8 |
| 32-A-C | Agata Klak (FS) def. Joanne Gao (SV) 6-4, 7-6 |
| 32-A-C | Gysell Daza Gutierre (SV) def. Nadege Koua (LSSU) 7-6, 6-3 |
| 32-A-C | Maegan Flight (UIS) def. Carolina Yepez (PNW) 6-2, 6-1 |
| 32-A-C | Constansa Crespo (SBU) def. Jennifer Skonieczny (BELL) 6-4, 6-2 |
| 32-A-C | Aleksandra Sredojevi (LU) def. Hinterberger, Sandra (MUW) 6-2, 2-6, 10-4 |
| 32-A-C | Neva Manas (MTW) def. Maddie Miller (SV) 6-3, 6-2 |
| 32-A-C | Luciana Pereyra (UF) def. AlexiAnn Drouin (LSSU) 6-3, 6-4 |
| 32-A-C | Danica Malan (DU) def. Aubrey Smith (BELL) 6-1, 7-6 |
| 32-A-C | Silvia Verebes (FS) def. Bascunana, Clara (MUW) 4-6, 6-4, 10-8 |
| 32-A-C | Nicole Beidacki (WALSHW) def. Taylor McLaughlin (SV) 7-5, 7-6 |
| 16-A-C | Linda Liong (WSUW) def. Emily Sim (TUWOMEN) 6-3, 6-4 |
| 16-A-C | Katie Bell (HCW) def. Joie Reisch (LU) 6-2, 6-1 |
| 16-A-C | Vera Griva (GV-W) def. Agata Klak (FS) 7-6, 6-3 |
| 16-A-C | Maegan Flight (UIS) def. Gysell Daza Gutierre (SV) 7-6, 5-7, 10-7 |
| 16-A-C | Aleksandra Sredojevi (LU) def. Constansa Crespo (SBU) 7-6, 4-6, 10-5 |
| 16-A-C | Neva Manas (MTW) def. Luciana Pereyra (UF) 6-2, 6-2 |
| 16-A-C | Danica Malan (DU) def. Silvia Verebes (FS) 6-4, 6-2 |
| 16-A-C | Nicole Beidacki (WALSHW) def. Olivia Kjaer (PNW) 6-2, 6-2 |
| QF-A-C | Linda Liong (WSUW) def. Katie Bell (HCW) 7-6, 6-4 |
| QF-A-C | Maegan Flight (UIS) def. Vera Griva (GV-W) 6-4, 6-4 |
| QF-A-C | Aleksandra Sredojevi (LU) def. Neva Manas (MTW) 2-6, 6-4, 10-4 |
| QF-A-C | Nicole Beidacki (WALSHW) def. Danica Malan (DU) 6-4, 6-2 |
| 32-B-C | Nicole Johnson (SV) def. Kayley Gimbel (UF) 6-4, 6-2 |
| 32-B-C | Evans, Abbie (CED) def. Elli Formentin (HCW) 6-0, 6-2 |
| 32-B-C | Samantha Johnson (MTW) def. Keegan Malpass (LSSU) 6-4, 1-6, 10-4 |
| 32-B-C | Dowden, Ashley (MUW) def. Vladislava Shumilova (DU) by default |
| 32-B-C | English, Janelle (CED) def. Ellie Chawner (HCW) 6-1, 6-1 |
| 32-B-C | Cupper, Georgina (MUW) def. Johanna Frye (FS) 6-4, 6-3 |
| 32-B-C | Clara Drasch (WALSHW) def. Emma Philippus (DU) 6-0, 6-0 |
| 32-B-C | Kyleigh Henthorn (WALSHW) def. English, Liana (CED) 6-4, 0-6, 10-2 |
| 32-B-C | Jasmine Cortez (UIS) def. Sara Neves (SV) 6-4, 7-5 |
| 32-B-C | Morgan Waller (FS) def. Agata Szewczuk (GV-W) 7-6, 7-5 |
| 32-B-C | Sophie Davis (LU) def. Sophie Gossett (DU) 6-4, 4-6, 10-6 |
| 32-B-C | Channing Varnum (BELL) def. Lillian Mouw (DU) 6-1, 6-1 |
| 32-B-C | Sofia Khairutdinova (WALSHW) def. Clara Lopez Bueno (UMSL) by default |
| 32-B-C | Sophia Spinazze (HCW) def. Madelyn Groulx (FS) 6-1, 6-0 |
| 32-B-C | Adiya Young (SV) def. Riley Gilman (LSSU) 6-1, 6-2 |
| 16-B-C | Evans, Abbie (CED) def. Nicole Johnson (SV) 6-3, 6-4, 12-10 |
| 16-B-C | Dowden, Ashley (MUW) def. Samantha Johnson (MTW) 6-3, 6-0 |
| 16-B-C | Cupper, Georgina (MUW) def. English, Janelle (CED) 6-1, 6-1 |
| 16-B-C | Clara Drasch (WALSHW) def. Bianca Buie (UMSL) 6-1, 6-0 |
| 16-B-C | Kyleigh Henthorn (WALSHW) def. Jasmine Cortez (UIS) 3-6, 6-4, 10-8 |
| 16-B-C | Morgan Waller (FS) def. Sophie Davis (LU) 6-4, 6-1 |
| 16-B-C | Channing Varnum (BELL) def. Sofia Khairutdinova (WALSHW) 6-4, 7-5 |
| 16-B-C | Sophia Spinazze (HCW) def. Adiya Young (SV) 4-6, 6-3, 11-9 |
| QF-B-C | Dowden, Ashley (MUW) def. Evans, Abbie (CED) 6-1, 6-3 |
| QF-B-C | Cupper, Georgina (MUW) def. Clara Drasch (WALSHW) 3-6, 6-0, 10-2 |
| QF-B-C | Morgan Waller (FS) def. Kyleigh Henthorn (WALSHW) 6-4, 6-2 |
| QF-B-C | Sophia Spinazze (HCW) def. Channing Varnum (BELL) 9-7 |
